Journal: Journal of Clinical Microbiology

Article Title: Designation of the Provisional New Enterococcus Species CDC PNS-E2 as Enterococcus sanguinicola sp. nov., Isolated from Human Blood, and Identification of a Strain Previously Named Enterococcus CDC PNS-E1 as Enterococcus italicus Fortina, Ricci, Mora, and Manachini 2004

doi: 10.1128/jcm.00603-08

Figure Lengend Snippet: FIG. 1. SDS-PAGE profiles of whole-cell protein extracts of Enterococcus sanguinicola sp. nov. (strains ATCC BAA-781T and CCUG 47884) and Enterococcus italicus (strains DSM 15952T and ATCC BAA-780). A dendrogram resulting from computer-assisted analysis of the protein profiles is shown on the left. MM, molecular mass marker (in kilodaltons).

Article Snippet: SDS-PAGE profiles of whole-cell protein extracts of Enterococcus sanguinicola sp. nov. (strains ATCC BAA-781T and CCUG 47884) and Enterococcus italicus (strains DSM 15952T and ATCC BAA-780).

Techniques: SDS Page, Marker

Journal: Journal of Clinical Microbiology

Article Title: Designation of the Provisional New Enterococcus Species CDC PNS-E2 as Enterococcus sanguinicola sp. nov., Isolated from Human Blood, and Identification of a Strain Previously Named Enterococcus CDC PNS-E1 as Enterococcus italicus Fortina, Ricci, Mora, and Manachini 2004

doi: 10.1128/jcm.00603-08

Figure Lengend Snippet: FIG. 2. Distance matrix tree based on 16S rRNA gene sequence comparisons showing the phylogenetic relationships among Enterococcus italicus, Enterococcus sanguinicola, and some of the most closely related species of Enterococcus. The Vagococcus fluvialis sequence was used as an outgroup. Bootstrap probability values (percentages of 1,000 tree replications) are given at branch points. Sequence accession numbers are given in parentheses after strain names.

Article Snippet: SDS-PAGE profiles of whole-cell protein extracts of Enterococcus sanguinicola sp. nov. (strains ATCC BAA-781T and CCUG 47884) and Enterococcus italicus (strains DSM 15952T and ATCC BAA-780).

Techniques: Sequencing

Journal: Journal of Clinical Microbiology

Article Title: Reevaluation of the Taxonomic Status of Recently Described Species of Enterococcus : Evidence that E. thailandicus Is a Senior Subjective Synonym of " E. sanguinicola " and Confirmation of E. caccae as a Species Distinct from E. silesiacus ^▿

doi: 10.1128/JCM.00399-11

Figure Lengend Snippet: Phenotypic characteristics of Enterococcus sanguinicola , Enterococcus thailandicus , Enterococcus caccae , Enterococcus silesiacus , and physiologically related species of Enterococcus

Article Snippet: Clinical laboratories should be aware on the occurrence of this additional enterococcal species potentially associated with vanA gene acquisition and transmission. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Source of unlabeled DNA Result with labeled DNA from a : E. sanguinicola ATCC BAA781 T E. caccae ATCC BAA-1240 T RBR at 55°C % D RBR at 70°C RBR at 55°C % D RBR at 70°C E. sanguinicola ATCC BAA-781 T 100 0.0 100 NP NP NP E. sanguinicola CCUG 47884 75 0.0 84 NP NP NP E. thailandicus NRBC 101867 T 73 0.5 77 NP NP NP E. caccae ATCC BAA-1240 T NP b NP NP 100 0.0 100 E. silesiacus LMG 23085 T NP NP NP 40 11.0 NP Open in a separate window a RBR, relative binding ratio; % D , percentage of divergence, calculated to the nearest 0.5%. b NP, not performed.

Techniques:

Journal: Journal of Clinical Microbiology

Article Title: Reevaluation of the Taxonomic Status of Recently Described Species of Enterococcus : Evidence that E. thailandicus Is a Senior Subjective Synonym of " E. sanguinicola " and Confirmation of E. caccae as a Species Distinct from E. silesiacus ^▿

doi: 10.1128/JCM.00399-11

Figure Lengend Snippet: Phylogenetic trees based on comparative analysis of the 16S rRNA (A) or rpoB (B) gene sequences, showing the relationships among the type strains of Enterococcus sanguinicola, Enterococcus thailandicus, Enterococcus caccae, Enterococcus silesiacus, and other selected species of Enterococcus. GenBank accession numbers are given in parentheses. The neighbor-joining method was used to create the dendrograms, and 1,000 resamplings at the nodes are displayed as percentages. Scale bars indicate numbers of substitutions per nucleotide position. V. fluvialis, Vagococcus fluvialis.

Article Snippet: Clinical laboratories should be aware on the occurrence of this additional enterococcal species potentially associated with vanA gene acquisition and transmission. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Source of unlabeled DNA Result with labeled DNA from a : E. sanguinicola ATCC BAA781 T E. caccae ATCC BAA-1240 T RBR at 55°C % D RBR at 70°C RBR at 55°C % D RBR at 70°C E. sanguinicola ATCC BAA-781 T 100 0.0 100 NP NP NP E. sanguinicola CCUG 47884 75 0.0 84 NP NP NP E. thailandicus NRBC 101867 T 73 0.5 77 NP NP NP E. caccae ATCC BAA-1240 T NP b NP NP 100 0.0 100 E. silesiacus LMG 23085 T NP NP NP 40 11.0 NP Open in a separate window a RBR, relative binding ratio; % D , percentage of divergence, calculated to the nearest 0.5%. b NP, not performed.

Techniques:

Journal: Journal of Clinical Microbiology

Article Title: Reevaluation of the Taxonomic Status of Recently Described Species of Enterococcus : Evidence that E. thailandicus Is a Senior Subjective Synonym of " E. sanguinicola " and Confirmation of E. caccae as a Species Distinct from E. silesiacus ^▿

doi: 10.1128/JCM.00399-11

Figure Lengend Snippet: Levels of DNA relatedness between Enterococcus sanguinicola and Enterococcus thailandicus and between Enterococcus caccae and Enterococcus silesiacus

Article Snippet: Clinical laboratories should be aware on the occurrence of this additional enterococcal species potentially associated with vanA gene acquisition and transmission. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Source of unlabeled DNA Result with labeled DNA from a : E. sanguinicola ATCC BAA781 T E. caccae ATCC BAA-1240 T RBR at 55°C % D RBR at 70°C RBR at 55°C % D RBR at 70°C E. sanguinicola ATCC BAA-781 T 100 0.0 100 NP NP NP E. sanguinicola CCUG 47884 75 0.0 84 NP NP NP E. thailandicus NRBC 101867 T 73 0.5 77 NP NP NP E. caccae ATCC BAA-1240 T NP b NP NP 100 0.0 100 E. silesiacus LMG 23085 T NP NP NP 40 11.0 NP Open in a separate window a RBR, relative binding ratio; % D , percentage of divergence, calculated to the nearest 0.5%. b NP, not performed.

Techniques: Labeling